Vibrio parahaemolyticus flagellin F (FlaF) induces the inflammatory response of the Tetraodon nigroviridis through the TLR5M

Vibrio parahaemolyticus , one of the major causes vibriosis in aquatic animals, inflammation that may be related to tissue damage. Flagellin is structural component flagellum, and also an important virulence factor bacteria. The filament V. flagellum composed multiple flagellin subunits, including FlaA, FlaB, FlaC, FlaD, FlaE, FlaF which are virulence. We produced six recombinant through prokaryotic expression r V.p FlaF. primary spleen head kidney cells (including leukocytes) Tetraodon nigroviridis were obtained, flagellins used as stimulants. In this study, on pro-inflammatory mediators release nitric oxide (NO) reactive oxygen species, well inflammatory cytokines IL-1β IFNγ2 etc detected. results showed NO production respiratory burst response significantly induced after stimulation with T. cells. Our mRNA levels toll-like receptor 5 M ( Tn TLR5M), TLR5S, downstream genes, such myeloid differentiation 88 (MyD88), TNF receptor-associated 6 (TRAF6), interleukin-1β (IL-1β), all enhanced addition, alkaline phosphatase (ALP) activity increased liver challenge vivo . Meanwhile, TLR5M-overexpressed HEK 293 T Cos-7 cells, luciferase nuclear factor-kappa B (NF-κB) reporter gene was stimulation, a time- dose-dependent manner. However, other five components have no inflammation-inducing ability or effect relatively weak. Overall, our suggested activates IFNγ2, TLR5M-NF-κB signaling pathway conclusion, study indicated has significant role inducing • induces activate immune TLR5M-MyD88-NF-κB pathway.